IL-31 Protein (AA 24-164)
Quick Overview for IL-31 Protein (AA 24-164) (ABIN1096936)
抗原
See all IL-31 (IL31) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 24-164
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原理
- Recombinant Human Interleukin-31/IL-31
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序列
- SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
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产品特性
- Recombinant Human Interleukin-31/IL-31 is produced with our E. coli expression system. The target protein is expressed with sequence (Ser24-Thr164) of Human IL-31.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Interleukin 31 (IL31) (AA 24-164) protein (His tag)
IL31 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 31 (IL31) (AA 27-163) protein (His tag)IL31 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 31 (IL31) (AA 24-159) protein (His tag)IL31 宿主: 犬 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
Interleukin 31 (IL31) (AA 24-163) protein (His tag)IL31 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 31 (IL31) (AA 24-164) protein (Fc Tag)IL31 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 31 (IL31) proteinIL31 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Interleukin 31 (IL31) (AA 24-164) (Active) protein (His tag)IL31 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 31 (IL31) proteinIL31 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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说明
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Biological activity: ED50 is 5 ng/mL as determined by inducing STAT3 activation in U87 MG human glioblastoma/astrocytoma cells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
有效期
- 3 months
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- IL-31 (IL31) (Interleukin 31 (IL31))
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别名
- il-31
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背景
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Human Interleukin 31 (IL-31) is a cytokine containing a four-helix bundle structure. It shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. Human IL-31 cDNA encodes a 164 amino acid precursor that contains a 23 amino acid signal peptide and a 141 amino acid mature protein. Human and mouse IL-31 share 24% sequence identity in the mature region. IL-31 is mainly associated with activated T cells and is preferentially expressed by type 2 helper T cells (Th2). IL-31 signals via a heterodimeric receptor complex composed of a gp130 related molecule termed IL-31RA (also GPL and GLMR) and an Oncostatin M receptor (OSM Rbeta). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFNgamma on monocytes. GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Studies have shown that IL31 induces severe pruritis (itching) and dermatitis in transgenic mice.
Alternative Names: Interleukin-31, IL-31, IL31 -
分子量
- 15.8 kDa
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UniProt
- Q6EBC2
抗原
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