CPA1 Protein (AA 17-419) (His tag)
Quick Overview for CPA1 Protein (AA 17-419) (His tag) (ABIN1096218)
抗原
See all CPA1 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 17-419
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标记
- This CPA1 protein is labelled with His tag.
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原理
- Recombinant Human Carboxypeptidase A1/CPA1 (C-6His)
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序列
- KEDFVGHQVL RISVADEAQV QKVKELEDLE HLQLDFWRGP AHPGSPIDVR VPFPSIQAVK IFLESHGISY ETMIEDVQSL LDEEQEQMFA FRSRARSTDT FNYATYHTLE EIYDFLDLLV AENPHLVSKI QIGNTYEGRP IYVLKFSTGG SKRPAIWIDT GIHSREWVTQ ASGVWFAKKI TQDYGQDAAF TAILDTLDIF LEIVTNPDGF AFTHSTNRMW RKTRSHTAGS LCIGVDPNRN WDAGFGLSGA SSNPCSETYR GKFANSEVEV KSIVDFVKDH GNIKAFISIH SYSQLLMYPY GYKTEPVPDQ DELDQLSKAA VTALASLYGT KFNYGSIIKA IYQASGSTID WTYSQGIKYS FTFELRDTGR YGFLLPASQI IPTAKETWLA LLTIMEHTLN HPYVDHHHHH H
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产品特性
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Recombinant Human Carboxypeptidase A1/CPA1 (C-6His)
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 1-419) protein (GST tag)
CPA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carboxypeptidase A1 (Pancreatic) (CPA1) (Active) protein (His tag)CPA1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
Carboxypeptidase A1 (Pancreatic) (CPA1) protein (Myc-DYKDDDDK Tag)CPA1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150mm NaCl, pH 7.5.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- -80 °C
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储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
有效期
- 6 months
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- CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))
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别名
- cpa1
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背景
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Recombinant Human Carboxypeptidase A1/CPA1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ala111-Tyr419) of Human CPA1 fused with a polyhistidine tag at the C-terminus.
Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E. -
分子量
- 46.57 kDa
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UniProt
- P15085
抗原
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