Annexin V Protein (DY-490)
Quick Overview for Annexin V Protein (DY-490) (ABIN1047953)
抗原
See all Annexin V (ANXA5) 蛋白蛋白类型
宿主
宿主
- 
    - 
                                            标记
- This Annexin V protein is labelled with DY-490.
- 
                                            产品特性
- 
                        Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
 Gene Name: Anxa5
- 
                                            试剂未包括
- 
                        Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2 
 Propidium iodide: 50 g/ml, final concentration 1 g/ml
 
- 
                                            
- 
    
- 
    - 
                                            应用备注
- 5 l are suitable for one assay
- 
                                            实验流程
- 
                        Staining protocol: 
 1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
 2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
 For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
 3. Incubate for 15min at room temperature in the dark
 4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour).
- 
                                            限制
- 仅限研究用
 
- 
                                            
- 
    - 
                                            状态
- Liquid
- 
                                            储存液
- Sodium azide
- 
                                            注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 
                                            储存条件
- 4 °C
 
- 
                                            
- 
    - 
                                            : "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V."    in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995)         (PubMed).        
 
 
- 
                                            : "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V."    in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995)         (PubMed).        
- 
    
- 
                    
 
                                     
                                     
                                    