Annexin V Protein (DY-490)
(1 reference)Quick Overview for Annexin V Protein (DY-490) (ABIN1047953)
抗原
See all Annexin V (ANXA5) 蛋白蛋白类型
宿主
宿主
-
-
标记
- This Annexin V protein is labelled with DY-490.
-
产品特性
-
Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5 -
试剂未包括
-
Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml
-
-
Annexin A5 (ANXA5) (AA 2-320) protein
ANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Annexin A5 (ANXA5) (AA 1-320) protein (GST tag)ANXA5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Annexin A5 (ANXA5) (AA 1-319) protein (His-EGFP Tag)ANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Annexin A5 (ANXA5) protein (FITC)ANXA5 宿主: 人 宿主: Bacteria Recombinant > 99 % (SDS-PAGE) FACS
Annexin A5 (ANXA5) (AA 1-320) proteinANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
-
-
应用备注
- 5 l are suitable for one assay
-
实验流程
-
Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour). -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
-
-
: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
-
: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
-
-
