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PLG ELISA 试剂盒

PLG 适用: 兔 Colorimetric Sandwich ELISA
产品编号 ABIN956091
发货至: 中国
  • 抗原 See all PLG ELISA试剂盒
    PLG (Plasminogen (PLG))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    The Rabbit Plasminogen ELISA is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for the quantitative determination of plasminogen in rabbit biological fluids.
    Analytical Method
    Quantitative
    产品特性
    In this assay the plasminogen present in the sample reacts with the anti-plasminogen antibody which has been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound sample proteins by washing, anti-plasminogen antibody conjugated with horseradish peroxidase (HRP) is added. This HRP-conjugated antibody forms a complex with the previously bound plasminogen. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme is proportional to the concentration of plasminogen in the sample tested, thus, the absorbance, at 450 nm, is a measure of the concentration of plasminogen in the test sample. The quantity of plasminogen in the test sample can be interpolated from the calibration curve constructed from the calibrators, and corrected for sample dilution.
    组件
    1. Diluent Concentrate: One bottle containing 50 mL of a 5X concentrated diluent running buffer.
    2. Wash Solution Concentrate: One bottle containing 50 mL of a 20X concentrated wash solution.
    3. Enzyme-Antibody Conjugate Concentrate: One vial containing 150 µL of a 100X concentrated affinity-purified anti-rabbit plasminogen antibody conjugated with HRP in a stabilizing buffer.
    4. TMB Substrate Solution: One vial containing 12 mL of TMB and hydrogen peroxide in citric acid buffer at pH 3.3.
    5. Stop Solution: One vial containing 12 mL of 0.3 M sulfuric acid. WARNING: Avoid contact with skin.
    6. Microtiter Plate: Twelve removable eight-well strips in well holder frame. Wells are coated with affinity-purified anti-rabbit plasminogen.
    7. Rabbit Plasminogen Calibrator: One vial containing a Rabbit Plasminogen Calibrator.
    8. Positive Control: One vial containing 50 µL of serum with 0.1% sodium azide. See the Control Certificate for the concentration.
    试剂未包括
    Precision pipettes (2 µL to 200 µL) for making and dispensing dilutions
    Test tubes
    Microplate washer/aspirator
    Distilled or de-ionized H2O Microplate reader
    Assorted glassware for the preparation of reagents and buffer solutions
    Timer
    Vortex mixer
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  • 板类型
    Pre-coated
    试剂准备
    1. Diluent Concentrate: The Diluent solution supplied is a 5X concentrate and must be diluted 1:5 with distilled or de-ionized water.
      2. Wash Solution Concentrate: The Wash Solution supplied is a 20X concentrate and must be diluted 1:20 with distilled or de-ionized water. Crystal formation in the concentrate is not uncommon when storage temperatures are low. Warming of the concentrate to 30-35°C before dilution can dissolve crystals.
      3. Enzyme-Antibody Conjugate Concentrate: Calculate the required amount of working conjugate solution for each microtiter plate test strip by adding 10 µL Enzyme-Antibody Conjugate to 990 µL of 1X Diluent for each test strip to be used for testing. Mix uniformly, but gently. Avoid foaming.
      4. TMB Substrate Solution: Ready to use as supplied.
      5. Stop Solution: Ready to use as supplied.
      6. Microtiter Plate: Ready to use as supplied. Unseal Microtiter Pouch and remove plate from pouch. Remove all strips and wells that will not be used in the assay and place back in pouch and re-seal along with desiccant.
      7. Rabbit Plasminogen Calibrator: The Rabbit Plasminogen Calibrator should be aliquoted out and stored frozen. It is at a concentration of 76.9 µg/mL and needs to be diluted in 1X Diluent according to the chart below for each run. Mix well between each step. Avoid foaming.
      8. Positive Control: The concentration and recommended dilution are provided on the Control Certificate. Before use, briefly centrifuge the Positive Control to allow all of the liquid to collect in the bottom of the vial.
    结果分析
    1. Subtract the average background value from the test values for each sample.
      2. Using the results observed for the calibrators construct a calibration curve. The appropriate curve fit is that of a four- parameter logistics curve. A second order polynomial (quadratic) or other curve fits may also be used.
      3. Interpolate test sample values from the calibration curve. Correct for sample dilution factor to arrive at plasminogen concentration in original sample.

      1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the information contained in the package insert instructions and with adherence to good laboratory practice.
      2. Factors that might affect the performance of the assay include proper instrument function, cleanliness of glassware, quality of distilled or de-ionized water, and accuracy of reagent and sample pipettings, washing technique, incubation time or temperature.
    限制
    仅限研究用
  • 储存条件
    4 °C
    储存方法
    1. Complete Kit: The expiration date for the kit is stated on the outer label. The recommended storage temperature is 4°C. Note: See long term storage recommendations below for the Rabbit Plasminogen Calibrator and Positive Control.
    2. Diluent: The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4°C.
    3. Wash Solution: The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (RT, 16-25°C) or at 4°C.
    4. Enzyme-Antibody Conjugate: Undiluted anti-plasminogen-HRP conjugate should be stored at 4°C and diluted immediately prior to use. The working conjugate solution is stable for up to eight hours at 4°C.
    5. TMB Substrate Solution: The TMB Substrate Solution should be stored at 4°C and is stable until the expiration date.
    6. Stop Solution: The Stop Solution should be stored at 4°C and is stable until the expiration date.
    7. Microtiter Plate: Anti-Rabbit plasminogen coated wells are stable until the expiration date and should be stored at 4°C in the sealed foil pouch with a desiccant pack.
    8. Rabbit Plasminogen Calibrator: Rabbit Plasminogen Calibrator should be aliquoted out and stored frozen. For storage longer than 14 days, keep frozen until the expiration date. Storage of less than 14 days can be at 4°C. Avoid multiple freeze/thaw cycles. The working calibrator solutions are stable for 12 hours.
    9. Positive Control: For storage longer than 7 days keep frozen until the expiration date.
    有效期
    The expiry date is stated on the label.
  • 抗原 See all PLG ELISA试剂盒
    PLG (Plasminogen (PLG))
    别名
    Plasminogen (PLG 产品)
    别名
    wu:fb70e09 ELISA Kit, PLG ELISA Kit, LPA ELISA Kit, plg ELISA Kit, Ab1-346 ELISA Kit, AI649309 ELISA Kit, Pg ELISA Kit, plasminogen ELISA Kit, plg ELISA Kit, PLG ELISA Kit, Plg ELISA Kit
    背景
    Plasminogen (PMG) is a glycoprotein produced by the liver. It is the precursor for plasmin, which targets fibrin in the process of dissolution of fibrin blood clots. Plasminogen is present in plasma and most extravascular fluids. The important role of plasminogen in fibrinolytic system makes it an interesting marker for various diseases.
    途径
    Complement System, Lipid Metabolism
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