Complement Factor H ELISA 试剂盒
-
- 抗原 See all Complement Factor H (CFH) ELISA试剂盒
- Complement Factor H (CFH)
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 ng/mL
- 最低检测浓度
- 15.6 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of mouse CF-H in serum, plasma and other biological fluids.
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of mouse CF-H. No significant cross- reactivity or interference was observed.
- 灵敏度
-
The minimum detectable dose of mouse CF-H is typically less than 6.7 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. - 产品特性
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to CF-H. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CF-H in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- 组件
-
Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - 试剂未包括
-
1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
- Discover our best selling CFH ELISA Kit
- Top Product
- Discover our top product CFH ELISA Kit
-
Complement Factor H (CFH) ELISA Kit
CFH 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 人 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 小鼠 AA 875-1252 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 大鼠 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Factor H (CFH) ELISA KitCFH 适用: 人 Colorimetric Sandwich ELISA 23.438 ng/mL - 1500 ng/mL Plasma, Serum, Tissue Homogenate
Complement Factor H (CFH) ELISA KitCFH 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Complement Factor H (CFH) ELISA KitCFH 适用: 人 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Factor H (CFH) ELISA KitWide Range CFH 适用: 人 Colorimetric Sandwich ELISA 23.44 ng/mL - 1500 ng/mL Plasma, Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 人 AA 860-1231 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
-
- 说明
-
The calibration curve concentrations used for the ELISA's were 1000 ng/mL, 500 ng/mL, 250 ng/mL, 125 ng/mL, 62.5 ng/mL, 31.2 ng/mL, 15.6 ng/mL.
- 板类型
- Pre-coated
- 结果分析
-
Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a calibration curve by plotting the mean absorbance for each calibrator on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CF-H concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. It is recommended to use some related software to do this calculation. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- 有效期
- The expiry date is stated on the label.
-
- 抗原 See all Complement Factor H (CFH) ELISA试剂盒
- Complement Factor H (CFH)
- 别名
- Complement Factor H (CF-H) (CFH 产品)
- 别名
- AHUS1 ELISA Kit, AMBP1 ELISA Kit, ARMD4 ELISA Kit, ARMS1 ELISA Kit, CFHL3 ELISA Kit, FH ELISA Kit, FHL1 ELISA Kit, HF ELISA Kit, HF1 ELISA Kit, HF2 ELISA Kit, HUS ELISA Kit, Mud-1 ELISA Kit, NOM ELISA Kit, Sas-1 ELISA Kit, Sas1 ELISA Kit, Fh ELISA Kit, CFH ELISA Kit, im:7147412 ELISA Kit, si:ch211-207o17.2 ELISA Kit, fH ELISA Kit, armd4 ELISA Kit, arms1 ELISA Kit, cfh ELISA Kit, cfhl3 ELISA Kit, fhl1 ELISA Kit, hf1 ELISA Kit, hf2 ELISA Kit, hus ELISA Kit, complement factor H ELISA Kit, complement component factor h ELISA Kit, complement factor H-related protein 5 ELISA Kit, complement factor H L homeolog ELISA Kit, CFH ELISA Kit, Cfh ELISA Kit, cfh ELISA Kit, LOC100399888 ELISA Kit, LOC100733902 ELISA Kit, cfh.L ELISA Kit
- 途径
- Complement System, Cellular Response to Molecule of Bacterial Origin
-
