C3a ELISA 试剂盒
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- 抗原 See all C3a ELISA试剂盒
- C3a (Complement Fragment 3a (C3a))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78-5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of mouse C3a in serum, plasma and other biological fluids.
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of mouse C3a. No significant cross- reactivity or interference between mouse C3a and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between mouse C3a and all the other analogues, therefore, cross reaction may still exist.
- 灵敏度
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The minimum detectable dose of mouse C3 a is typically less than 29 pg/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. - 产品特性
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3a. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of C3a in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- 组件
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Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - 试剂未包括
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1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- Discover our top product C3a ELISA Kit
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Complement Fragment 3a (C3a) ELISA Kit
C3a 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a 适用: 绵羊 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a 适用: 猴 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Fragment 3a (C3a) ELISA KitWide Range C3a 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a 适用: 兔 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Complement Fragment 3a (C3a) ELISA KitC3a 适用: 犬 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Fragment 3a (C3a) ELISA KitC3a 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Complement Fragment 3a (C3a) ELISA KitC3a 适用: 兔 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The calibration curve concentrations used for the ELISA's were 5, 000 pg/mL, 2, 500 pg/mL, 1250 pg/mL, 625 pg/mL, 312 pg/mL, 156 pg/mL, 78 pg/mL.
- 板类型
- Pre-coated
- 结果分析
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Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with C3a concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- 有效期
- The expiry date is stated on the label.
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- 抗原 See all C3a ELISA试剂盒
- C3a (Complement Fragment 3a (C3a))
- 别名
- C3a (C3a 产品)
- 别名
- AHUS5 ELISA Kit, ARMD9 ELISA Kit, ASP ELISA Kit, C3a ELISA Kit, C3b ELISA Kit, CPAMD1 ELISA Kit, AI255234 ELISA Kit, HSE-MSF ELISA Kit, Plp ELISA Kit, C3d ELISA Kit, complement C3 ELISA Kit, complement component 3 ELISA Kit, C3 ELISA Kit
- 途径
- Complement System
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