Prothrombin ELISA 试剂盒
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- 抗原 See all Prothrombin (F2) ELISA试剂盒
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156-10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of mouse F2 in plasma.
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of mouse F2. No significant cross- reactivity or interference was observed.
- 灵敏度
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The minimum detectable dose of mouse F2 is typically less than 0.041 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. - 产品特性
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to F2. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of F2 in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- 组件
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Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - 试剂未包括
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1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Coagulation Factor II (thrombin) (F2) ELISA Kit
F2 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 人 Colorimetric Sandwich ELISA 9.38 ng/mL - 600 ng/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 人 Colorimetric Sandwich ELISA 0.41 ng/mL - 300 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 人 Fragment 1 Colorimetric Sandwich ELISA 0.03-2 nM/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The calibration curve concentrations used for the ELISA's were 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL.
- 板类型
- Pre-coated
- 结果分析
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Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a calibration curve by plotting the mean absorbance for each calibrator on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the F2 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. It is recommended to use some related software to do this calculation. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- 有效期
- The expiry date is stated on the label.
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- 抗原 See all Prothrombin (F2) ELISA试剂盒
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- 别名
- F2 (F2 产品)
- 别名
- PT ELISA Kit, RPRGL2 ELISA Kit, THPH1 ELISA Kit, Cf-2 ELISA Kit, Cf2 ELISA Kit, FII ELISA Kit, thrombin ELISA Kit, zgc:66319 ELISA Kit, wu:fb57c10 ELISA Kit, wu:fd42e09 ELISA Kit, wu:fd59d01 ELISA Kit, prothrombin ELISA Kit, F2 ELISA Kit, coagulation factor II, thrombin ELISA Kit, coagulation factor II ELISA Kit, coagulation factor II (thrombin) ELISA Kit, F2 ELISA Kit, f2 ELISA Kit
- 途径
- Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
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