Gliadin Screen ELISA 试剂盒
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- 抗原
- Gliadin Screen
- 适用
- 小麦胚
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- 产品特性
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ELISA kit for the detection of Gliadin screen in the research laboratory
Alternative Names: Gliadin ELISA kit
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- 应用备注
- Optimal conditions to be determined by end-user
- 板类型
- Pre-coated
- 实验流程
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Purified gliadin from wheat is bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated antihuman IgG and antihuman IgA immunologically detect the patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. Anenzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG and IgA antibodies present in the original sample.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原
- Gliadin Screen
- 背景
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Gliadins are proteins containing high amounts of the amino acids prolin and glutamine. This protein belongs to the nutritive tissue of the grain seeds of wheat, oat, barley and rye and is responsible for the baking properties of the flour. Due to the possibilities of the highly specific and sensitive serological determination of IgA and IgG antibodies against gliadin the invasive procedures of biopsies can be given up. In the past several biopsies have been done with patients when celiac disease was suspected, after a period of a gluten-free diet and also after a specific gluten challenge Gliadin antibodies titer correlate very well with the morphological appearance of the mucosa of the upper intestine. It has been well documented that gliadin antibodies level fall very quickly after a gluten free diet has begun and rise immediately after restoring gluten to the diet. Thus the serological test represents a reliable method to monitor patients, and in particular children and teenagers, for their adherence to the glutenfree diet.
Synonyms: Gliadin ELISA kit.
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