Cathepsin G ELISA 试剂盒
Quick Overview for Cathepsin G ELISA 试剂盒 (ABIN930711)
抗原
See all Cathepsin G (CTSG) ELISA试剂盒适用
检测方法
实验类型
应用范围
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Analytical Method
- Quantitative
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产品特性
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ELISA kit for the detection of Cathepsin G in the research laboratory
Alternative Names: Cathepsin G ELISA kit
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Cathepsin G (CTSG) ELISA Kit
CTSG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin G (CTSG) ELISA KitCTSG 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin G (CTSG) ELISA KitCTSG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin G (CTSG) ELISA KitCTSG 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Cathepsin G (CTSG) ELISA KitHigh Sensitivity CTSG 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
SensoLyte® 520 Cathepsin G Assay KitFRET Fluorometric
Cathepsin G (CTSG) ELISA KitCTSG 适用: 人 Colorimetric Sandwich ELISA 3.125-100 ng/mL Cell Culture Cells, Plasma, Saliva, Serum
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应用备注
- Optimal conditions to be determined by end-user
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板类型
- Pre-coated
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实验流程
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Highly purified Cathepsin G is bound to microwells . Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- Cathepsin G (CTSG)
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别名
- Cathepsin G
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背景
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The cathepsins belong to a group of intracellular proteases mainly found in lysosomes, especially of the spleen, the liver and the kidney. Cathepsin G is a serine protease and a further pANCA antigen. It participates in a great part in the destruction of osteid tissue as a result of its hydrolytic properties. The autoantibodies against Cathepsin G occur mainly in collagenosis and other related inflammatory rheumatic diseases, e.g. SLE, Sjögren syndrome and Felty syndrome.
Synonyms: Cathepsin G ELISA kit. -
途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
抗原 See all Cathepsin G (CTSG) ELISA试剂盒
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