Dopamine ELISA 试剂盒
Quick Overview for Dopamine ELISA 试剂盒 (ABIN930670)
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实验类型
应用范围
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产品特性
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ELISA kit for the detection of Dopamine in the research laboratory
Alternative Names: Dopamine ELISA kit
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Dopamine (DA) ELISA Kit
DA 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Dopamine (DA) ELISA KitDA 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Dopamine (DA) ELISA KitDA 适用: Various Species Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Dopamine (DA) ELISA KitDA 适用: 小鼠 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Dopamine (DA) ELISA KitDA 适用: Various Species Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Dopamine (DA) ELISA KitDA 适用: 化学剂 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Dopamine (DA) ELISA KitDA 适用: 人 Colorimetric Competition ELISA 0.5-100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Dopamine (DA) ELISA KitDA 适用: 鱼 Colorimetric 62.5-1000 pg/mL Plasma, Serum, Tissue Homogenate
Dopamine (DA) ELISA KitDA 适用: Pig Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal conditions to be determined by end-user
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实验流程
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Dopamine is extracted by using a cisdiolspecific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- Dopamine (DA)
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别名
- Dopamine
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物质类
- Chemical
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背景
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Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptor (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses.
Synonyms: Dopamine ELISA kit.
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