Prostate Specific Antigen ELISA 试剂盒
Quick Overview for Prostate Specific Antigen ELISA 试剂盒 (ABIN921102)
抗原
See all Prostate Specific Antigen (PSA) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallikrein 3
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: A18-P261 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: A18-P261
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Prostate Specific Antigen (PSA) ELISA Kit
PSA 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prostate Specific Antigen (PSA) ELISA KitPSA 适用: 人 Colorimetric Sandwich ELISA 0.2 ng/mL - 12.5 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prostate Specific Antigen (PSA) ELISA KitPSA 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Prostate Specific Antigen (PSA) ELISA KitPSA 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Prostate Specific Antigen (PSA) ELISA KitPSA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.
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板类型
- Pre-coated
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实验流程
- human Kallikrein 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein 3 has been precoated onto 96-well plates. Standards(NSO, A18-P261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein 3 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Kallikrein 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Kallikrein 3 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.8, Standard deviation: 0.108, CV(%): 6
- Sample 2: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.422, CV(%): 6.3
- Sample 3: n=16, Mean(ng/ml): 12.7, Standard deviation: 0.686, CV(%): 5.4,
- Sample 1: n=24, Mean(ng/ml): 2.6, Standard deviation: 0.203, CV(%): 7.8
- Sample 2: n=24, Mean(ng/ml): 8.4, Standard deviation: 0.58, CV(%): 6.9
- Sample 3: n=24, Mean(ng/ml): 14.7, Standard deviation: 0.853, CV(%): 5.8
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Prostate Specific Antigen (PSA)
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别名
- KLK3
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背景
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Protein Function: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Background: Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland, PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3
Synonyms: Prostate-specific antigen,PSA,3.4.21.77,Gamma-seminoprotein,Seminin,Kallikrein-3,P-30 antigen,Semenogelase,KLK3,APS,
Full Gene Name: Prostate-specific antigen
Cellular Localisation: Secreted. -
基因ID
- 354
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UniProt
- P07288
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途径
- Complement System
抗原 See all Prostate Specific Antigen (PSA) ELISA试剂盒
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