LGALS1/Galectin 1 ELISA 试剂盒
Quick Overview for LGALS1/Galectin 1 ELISA 试剂盒 (ABIN921097)
抗原
See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse GALECTIN-1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: A2-E135 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <5pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: A2-E135
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Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitSmall Sample LGALS1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: Cow Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 人 AA 2-135 Colorimetric Sandwich ELISA
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 小鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 galectin domain.
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板类型
- Pre-coated
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实验流程
- mouse Galectin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-1 has been precoated onto 96-well plates. Standards(E.coli, A2-E135) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Galectin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Galectin-1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 501, Standard deviation: 22.04, CV(%): 4.4
- Sample 2: n=16, Mean(pg/ml): 3235, Standard deviation: 148.8, CV(%): 4.6
- Sample 3: n=16, Mean(pg/ml): 5618, Standard deviation: 309, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 521, Standard deviation: 30.22, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 3547, Standard deviation: 223.5, CV(%): 6.3
- Sample 3: n=24, Mean(pg/ml): 5296, Standard deviation: 397.2, CV(%): 7.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
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别名
- LGALS1
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背景
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Protein Function: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity. Strong inducer of T-cell apoptosis (By similarity). .
Background: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.
Synonyms: Galectin-1,Gal-1,14 kDa lectin,Beta-galactoside-binding lectin L-14-I,Galaptin,Lactose-binding lectin 1,Lectin galactoside-binding soluble 1,S-Lac lectin 1,Lgals1,Gbp,
Full Gene Name: Galectin-1
Cellular Localisation: Secreted, extracellular space, extracellular matrix. -
基因ID
- 16852
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UniProt
- P16045
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途径
- Carbohydrate Homeostasis
抗原 See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒
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