LGALS1/Galectin 1 ELISA 试剂盒
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北京 101111
Quick Overview for LGALS1/Galectin 1 ELISA 试剂盒 (ABIN5510585)
抗原
See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human Galectin-1 ELISA Kit to quantitate Human LGALS1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: A2-D135Capture antibody and Detection antibody: A polyclonal antibody from goat, a biotinylated detection polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 15 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitLGALS1 适用: Cow Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA KitSmall Sample LGALS1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5.8%, 6.8%, 8.1%
Inter-Assay Precision (CV%): 6.1%, 8.1%, 8.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma." in: Oncotarget, Vol. 7, Issue 35, pp. 57099-57116, (2018) (PubMed).
: "Galectin-1 from cancer-associated fibroblasts induces epithelial-mesenchymal transition through β1 integrin-mediated upregulation of Gli1 in gastric cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 175, (2017) (PubMed).
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- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
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别名
- LGALS1
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背景
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Background: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.
Gene Full Name: galectin 1
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基因ID
- 3956
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UniProt
- P09382
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途径
- Carbohydrate Homeostasis
抗原 See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒
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