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Cathepsin B ELISA 试剂盒

This 小鼠 Cathepsin B ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Cathepsin B.
产品编号 ABIN921085
发货至: 中国

Quick Overview for Cathepsin B ELISA 试剂盒 (ABIN921085)

抗原

See all Cathepsin B (CTSB) ELISA试剂盒
Cathepsin B (CTSB)

抗原表位

AA 18-339

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156-10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • 最低检测浓度

    156 pg/mL

    原理

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cathepsin B

    品牌

    PicoKine™

    Analytical Method

    Quantitative

    特异性

    Expression system for standard: NSO
    Immunogen sequence: H18-F339

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    <5pg/mL

    试剂未包括

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    免疫原

    Expression system for standard: NSO
    Immunogen sequence: H18-F339
  • 应用备注

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    板类型

    Pre-coated

    实验流程

    mouse Cathepsin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cathepsin B has been precoated onto 96-well plates. Standards(NSO, H18-F339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cathepsin B amount of sample captured in plate.

    实验流程

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Cathepsin B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Cathepsin B standard solution and each sample be measured in duplicate.

    实验精密度

    • Sample 1: n=16, Mean(pg/ml): 1154, Standard deviation: 61.2, CV(%): 5.3
    • Sample 2: n=16, Mean(pg/ml): 2875, Standard deviation: 161.0, CV(%): 5.6
    • Sample 3: n=16, Mean(pg/ml): 5823, Standard deviation: 355.2, CV(%): 6.1,
    • Sample 1: n=24, Mean(pg/ml): 1298, Standard deviation: 102.5, CV(%): 7.9
    • Sample 2: n=24, Mean(pg/ml): 2794, Standard deviation: 231.9, CV(%): 8.3
    • Sample 3: n=24, Mean(pg/ml): 5940, Standard deviation: 528.7, CV(%): 8.9

    限制

    仅限研究用
  • 注意事项

    Avoid multiple freeze-thaw cycles.

    储存条件

    -20 °C,4 °C

    储存方法

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    有效期

    12 months
  • 抗原 See all Cathepsin B (CTSB) ELISA试剂盒

    Cathepsin B (CTSB)

    别名

    CTSB

    背景

    Protein Function: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

    Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.

    Synonyms: Cathepsin B,3.4.22.1,Cathepsin B1,Cathepsin B light chain,Cathepsin B heavy chain,Ctsb,

    Full Gene Name: Cathepsin B

    Cellular Localisation: Lysosome. Melanosome . Secreted, extracellular space.

    基因ID

    13030

    UniProt

    P10605

    途径

    Activation of Innate immune Response, Toll-Like Receptors Cascades
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