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Galectin 3 ELISA 试剂盒

Pig Galectin 3 ELISA Kit, Colorimetric is an assay for quantification of Pig Galectin 3.
产品编号 ABIN8126255
发货至: 中国
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Quick Overview for Galectin 3 ELISA 试剂盒 (ABIN8126255)

抗原

See all Galectin 3 (LGALS3) ELISA试剂盒
Galectin 3 (LGALS3)

适用

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Pig

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Plasma, Serum
  • 原理

    This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

    Analytical Method

    Quantitative

    产品特性

    This immunoassay kit allows for the in vitro quantitative determination of Porcine Gal 3 (Galectin 3) concentrations in serum, plasma. Detection Range: 0.156-10 ng/mL Sensitivity: <0.094 ng/mL
  • 板类型

    Pre-coated

    实验流程

    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GAL3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GAL3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GAL3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GAL3 can be calculated.

    限制

    仅限研究用
  • 储存方法

    Please refer to the Manual
  • 抗原 See all Galectin 3 (LGALS3) ELISA试剂盒

    Galectin 3 (LGALS3)

    别名

    GAL3 (Galectin 3)

    途径

    RTK signaling
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