VEGFA ELISA 试剂盒
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Quick Overview for VEGFA ELISA 试剂盒 (ABIN8126240)
抗原
See all VEGFA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate.
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Analytical Method
- Quantitative
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灵敏度
- 9.0 pg/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human VEGF concentrations in serum, plasma. Detection Range: 31.2 - 2000 pg/mL Sensitivity: < 9.0 pg/mL
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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储存方法
- Please refer to the Manual
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGF (Vascular Endothelial Cell Growth Factor)
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原 See all VEGFA ELISA试剂盒
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