CXCL9 ELISA 试剂盒
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北京 101111
Quick Overview for CXCL9 ELISA 试剂盒 (ABIN8126235)
抗原
See all CXCL9 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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灵敏度
- 94 pg/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human CXCL9 concentrations in serum, plasma. Detection Range: 156 - 10,000 pg/mL Sensitivity: <94 pg/mL
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human CXCL9 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human CXCL9 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CXCL9 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human CXCL9 can be calculated using the standard curve.
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限制
- 仅限研究用
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储存方法
- Please refer to the Manual
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- CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
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别名
- CXCL9/MIG (C-X-C motif chemokine 9)
抗原 See all CXCL9 ELISA试剂盒
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