电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

CXCL9 ELISA 试剂盒

CXCL9 适用: 人 AA 23-125 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN411395
发货至: 中国
  • 抗原 See all CXCL9 ELISA试剂盒
    CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
    抗原表位
    AA 23-125
    适用
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2-2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL9
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: E.coli
    Immunogen sequence: T23-T125
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <2pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: E.coli
    Immunogen sequence: T23-T125
    Featured
    Discover our best selling CXCL9 ELISA Kit
    Top Product
    Discover our top product CXCL9 ELISA Kit
  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

    板类型
    Pre-coated
    实验流程
    human CXCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL9 has been precoated onto 96-well plates. Standards(E.coli, T23-T125) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL9 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL9 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 249, Standard deviation: 11.5, CV(%): 4.6
    • Sample 2: n=16, Mean(pg/ml): 834, Standard deviation: 35.03, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 1573, Standard deviation: 64.5, CV(%): 4.1,
    • Sample 1: n=24, Mean(pg/ml): 231, Standard deviation: 14.1, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 759, Standard deviation: 63.8, CV(%): 8.4
    • Sample 3: n=24, Mean(pg/ml): 1628, Standard deviation: 117.2, CV(%): 7.2
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all CXCL9 ELISA试剂盒
    CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
    别名
    CXCL9 (CXCL9 产品)
    别名
    CMK ELISA Kit, Humig ELISA Kit, MIG ELISA Kit, SCYB9 ELISA Kit, crg-10 ELISA Kit, BB139920 ELISA Kit, Mig ELISA Kit, MuMIG ELISA Kit, Scyb9 ELISA Kit, C-X-C motif chemokine ligand 9 ELISA Kit, chemokine (C-X-C motif) ligand 9 ELISA Kit, CXCL9 ELISA Kit, Cxcl9 ELISA Kit
    背景

    Protein Function: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.

    Background: Chemokine(C-X-C motif) ligand 9(CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon(MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

    Synonyms: C-X-C motif chemokine 9,Gamma-interferon-induced monokine,Monokine induced by interferon-gamma,HuMIG,MIG,Small-inducible cytokine B9,CXCL9,CMK, MIG, SCYB9,

    Full Gene Name: C-X-C motif chemokine 9

    Cellular Localisation: Secreted.
    基因ID
    4283
    UniProt
    Q07325
You are here:
客服