Interferon gamma ELISA 试剂盒
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Quick Overview for Interferon gamma ELISA 试剂盒 (ABIN8090635)
抗原
See all Interferon gamma (IFNG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 2.34 pg/mL
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原理
- Human HS-IFN-γ (High sensitive Interferon gamma) high sensitive Accquant ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with IFN-γ , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (The matrix components in serum/plasma will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing! When the content of other samples is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy serum (n=21) 1/2 dilution ND-22pg/mL Healthy plasma (EDTA, Citrate , heparin) (n=21) 1/2 dilution ND-16pg/mL Human peripheral blood lymphocyte were cultured with 5 %FBS + 1640 + double antibody+1 μg/mL LPS, and the cell culture supernatant was detected after 12 hours. 1/200-1/500 dilution 14.6 ng/mL Human peripheral blood lymphocyte were cultured with 5 %FBS + 1640 + double antibody+10 μg/mL PHA, and the cell culture supernatant was detected after 12 hours. 1/2 dilution 250pg/mL Human peripheral blood lymphocyte were treated with 10 μg/mL PHA for 12 hours. After that, 300 ng/mL Brefeldin A (BFA) was added and cultured for 3 hours. The cell lysate (Cat No: E050) was collected, and the total protein concentration of 1.56 mg/mL was detected by BCA. 1/10 dilution 1.15 ng/mg(total protein) 2. KO sample validation (Detect IFN-γ KO Jurkat cells): Sample Type Dilution Ratio Content Wild Jurkat cells were stimulated with 50 ng/mL PMA and 1 μM ionomycin for 12 hours to detect cell culture supernatant 1/5 dilution 310pg/mL KO Jurkat cells were stimulated with 50 ng/mL PMA and 1 μM ionomycin for 12 hours to detect cell culture supernatant 1/2 dilution ND KO Jurkat cells were treated with 50 ng/mL PMA and 1 μM ionomycin for 12 hours and then cultured with 300 ng/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 2.23 mg/mL) to detect. 1/2 dilution ND Note:ND is lower than the sensitivity of the kit and was not detected 3. Capture antibody and detection antibody by WB KO validation (Detect IFN-γ KO Jurkat cells):
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灵敏度
- 1.41 pg/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Biotin-labeled Antibody
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Interferon gamma (IFNG) ELISA Kit
IFNG 适用: 小鸡 Colorimetric Competition ELISA 3.125 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鼠 AA 23-155 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum
Interferon gamma (IFNG) IQ-ELISA KitIFNG 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 豚鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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实验时间
- 5 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- Interferon gamma (IFNG)
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别名
- IFN-gamma
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背景
- Interferon gamma, IFN-gamma, Immune interferon, IFNG
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UniProt
- P01579
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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