Angiotensin II ELISA 试剂盒
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Quick Overview for Angiotensin II ELISA 试剂盒 (ABIN8089438)
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See all Angiotensin II (Ang II) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.563 pg/mL
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原理
- Bovine Angiotensin II (Angiotensin II) ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with ANGII , no obvious cross reaction with other analogues.
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灵敏度
- 0.938 pg/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- HRP-Antigen(Concentrated
- 100X)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Angiotensin II (Ang II) ELISA Kit
Ang II 适用: 大鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II 适用: 小鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II 适用: 大鼠 Colorimetric Sandwich ELISA 4.7 pg/mL - 300 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II 适用: 人 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II 适用: 犬 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum
Angiotensin II (Ang II) ELISA KitAng II 适用: 人 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II 适用: 兔 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II 适用: 大鼠 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Angiotensin II (Ang II) ELISA KitAng II 适用: 马 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Competitive ELISA, Coated with Antibody
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实验时间
- 1 h
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板类型
- Pre-coated
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实验流程
- Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard/sample/HRP-labeled antigen working solution into each well. Seal the plate and statically incubate for 45 minutes at 37 °C. (Equilibrate the whole bottle of TMB for 30 min at 37 °C.) Washing: Wash the plate five times and immerse for 1 min each time. Step 3: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- Angiotensin II (Ang II)
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别名
- ANG II
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背景
- ANG II, Angiotensin II
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UniProt
- P01017
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途径
- JAK/STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
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