PKC alpha ELISA 试剂盒
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Quick Overview for PKC alpha ELISA 试剂盒 (ABIN8085846)
抗原
See all PKC alpha (PKCa) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- PKC α Colorimetric Cell-Based ELISA Kit for detecting PKC α in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Protein Kinase C, alpha (PKCa) ELISA Kit
PKCa 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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实验时间
- 4 - 5 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C for one month, Long term: see manual.
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有效期
- 6 months
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- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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别名
- PKC alpha
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背景
- Gene Aliases: kinase PKC-alpha, KPCA, PKC III, PKC-A, PKC-alpha, PKC-III, PRKACA, PRKCA, Protein kinase C alpha type
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基因ID
- 5578
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UniProt
- P17252
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途径
- WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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