GRB2 ELISA 试剂盒
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北京 101111
Quick Overview for GRB2 ELISA 试剂盒 (ABIN8080240)
抗原
See all GRB2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.312 ng/mL
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原理
- Mouse Growth Factor Receptor Bound Protein 2 (Grb2) ELISA Kit for detecting Grb2 in tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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灵敏度
- 0.113 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验精密度
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C for one month, Long term: see manual.
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有效期
- 12 months
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- GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
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别名
- Growth Factor Receptor Bound Protein 2
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背景
- Gene Aliases: ASH, NCKAP2, EGFRBP-GRB2, Grb3-3, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2
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基因ID
- 14784
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UniProt
- Q60631
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途径
- RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation
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