TRIM21 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 TRIM21.
产品编号 ABIN8071167
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for TRIM21 ELISA 试剂盒 (ABIN8071167)
抗原
See all TRIM21 ELISA试剂盒
TRIM21
(Tripartite Motif Containing 21 (TRIM21))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- Sjogren Syndrome Antigen (SSA) Human IgM ELISA Kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgM autoantibody against SSA in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.
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Analytical Method
- Quantitative
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组件
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- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- Sample Diluent
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- TRIM21 (Tripartite Motif Containing 21 (TRIM21))
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别名
- TRIM21
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背景
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Gene Symbols: TRIM21 RNF81 RO52 SSA1
Protein Name (Synonyms): E3 ubiquitin-protein ligase TRIM21 (EC 2.3.2.27) (52 kDa Ro protein) (52 kDa ribonucleoprotein autoantigen Ro/SS-A) (RING finger protein 81) (Ro(SS-A)) (Sjoegren syndrome type A antigen) (SS-A) (Tripartite motif-containing protein 21)
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基因ID
- 6737
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UniProt
- Q5XPV5, Q96RF8
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