TRIM21 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for TRIM21 ELISA 试剂盒 (ABIN8071161)
抗原
See all TRIM21 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 1.37 units/mL
-
原理
- The Sjogren Syndrome Antigen (SSA) Human IgA ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against SSA antigen(s) in human serum or plasma.
-
Analytical Method
- Quantitative
-
组件
-
- Size / Description
- SSA Coated 96-Well Microplate
- 96 wells coated with SSA
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgA
- HRP-Streptavidin
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent
- Sample Diluent
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
-
-
-
-
应用备注
- Suggested Plasma Dilution: 1,000-fold
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated Anti-Human IgA Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The unopened kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles.
-
-
- TRIM21 (Tripartite Motif Containing 21 (TRIM21))
-
别名
- TRIM21
-
背景
-
Gene Symbols: TRIM21 RNF81 RO52 SSA1
Background: The Sjogren Syndrome Antigen (SSA) Human IgA ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with SSA protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-SSA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): E3 ubiquitin-protein ligase TRIM21 (EC 2.3.2.27) (52 kDa Ro protein) (52 kDa ribonucleoprotein autoantigen Ro/SS-A) (RING finger protein 81) (Ro(SS-A)) (Sjoegren syndrome type A antigen) (SS-A) (Tripartite motif-containing protein 21)
-
基因ID
- 6737
-
UniProt
- Q5XPV5, Q96RF8
抗原 See all TRIM21 ELISA试剂盒
-
