TRIM21 ELISA 试剂盒
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北京 101111
Quick Overview for TRIM21 ELISA 试剂盒 (ABIN8071164)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 4.096 units/mL
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原理
- The Sjogren Syndrome Antigen (SSA) Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against pathogen antigen(s) in human serum or plasma.
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Analytical Method
- Quantitative
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组件
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- Size / Description
- SSA Coated 96-Well Microplate
- 96 wells coated with SSA
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgA
- HRP-Streptavidin
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent
- Sample Diluent
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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应用备注
- Suggested Plasma Dilution: 1000-fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated Anti-Human IgA Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- TRIM21 (Tripartite Motif Containing 21 (TRIM21))
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别名
- TRIM21
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背景
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Gene Symbols: TRIM21 RNF81 RO52 SSA1
Background: The Sjogren Syndrome Antigen (SSA) Human IgG ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with a pathogen antigen, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control consists of an anti-Sjogren Syndrome Antigen (SSA) antibody. A biotinylated anti-Positive Control antibody is added to the Positive Control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.
Protein Name (Synonyms): E3 ubiquitin-protein ligase TRIM21 (EC 2.3.2.27) (52 kDa Ro protein) (52 kDa ribonucleoprotein autoantigen Ro/SS-A) (RING finger protein 81) (Ro(SS-A)) (Sjoegren syndrome type A antigen) (SS-A) (Tripartite motif-containing protein 21)
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基因ID
- 6737
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UniProt
- Q5XPV5, Q96RF8
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