MAPT ELISA 试剂盒
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Quick Overview for MAPT ELISA 试剂盒 (ABIN8070981)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.37 ng/mL
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原理
- Phospho-TAU (Thr217) Quantitative ELISA Kit is a convenient and sensitive assay to detect phosphorylated Tau at Threonine-217 (p-Tau217) in human cell lysate samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human, mouse and rat Phospho TAU (Thr217).
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灵敏度
- 0.09 ng/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Microtubule-Associated Protein tau (MAPT) ELISA Kit
MAPT 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cerebrospinal Fluid, Plasma, Serum
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 大鼠 phosphorylated Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 人 phosphorylated Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cerebrospinal Fluid, Plasma, Serum
Microtubule-Associated Protein tau (MAPT) ELISA KitMAPT 适用: 人 Phosphospecific Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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- MAPT (Microtubule-Associated Protein tau (MAPT))
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别名
- Tau
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背景
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Gene Symbols: MAPT MAPTL MTBT1 TAU
Protein Name (Synonyms): Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)
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基因ID
- 4137
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UniProt
- P10636
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途径
- MAPK Pathway, Microtubule Dynamics, M Phase, Regulation of Cell Size
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