PDGFRA ELISA 试剂盒
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Quick Overview for PDGFRA ELISA 试剂盒 (ABIN8070454)
抗原
See all PDGFRA ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
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原理
- Phospho-PDGFRa (Tyr762) ELISA Kit measures phosphorylated PDGFRa (Tyr762) and total (pan) PDGFRa in cell and tissue lysates.
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Analytical Method
- Semi-Quantitative
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA Kit
PDGFRA 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 人 AA 24-524 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Platelet Derived Growth Factor Receptor alpha (PDGFRA) ELISA KitPDGFRA 适用: 人 Colorimetric Sandwich ELISA 0.4 ng/mL - 120 ng/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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- PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))
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别名
- PDGF R alpha
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背景
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Gene Symbols: PDGFRA PDGFR2 RHEPDGFRA
Protein Name (Synonyms): Platelet-derived growth factor receptor alpha (PDGF-R-alpha) (PDGFR-alpha) (EC 2.7.10.1) (Alpha platelet-derived growth factor receptor) (Alpha-type platelet-derived growth factor receptor) (CD140 antigen-like family member A) (CD140a antigen) (Platelet-derived growth factor alpha receptor) (Platelet-derived growth factor receptor 2) (PDGFR-2) (CD antigen CD140a)
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基因ID
- 5156
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UniProt
- P16234
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling
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