Caspase 4 ELISA 试剂盒
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Quick Overview for Caspase 4 ELISA 试剂盒 (ABIN8068191)
抗原
See all Caspase 4 (CASP4) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human Caspase-4 ELISA kit for cell culture supernatants, serum, and plasma samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair recognizes Human Caspase-4.
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) ELISA Kit
CASP4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) ELISA KitCASP4 适用: 小鼠 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) ELISA KitCASP4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) ELISA KitCASP4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) ELISA KitCASP4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Samples, Tissue Lysate
Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) ELISA KitCASP4 适用: 小鼠 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Cell Samples, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))
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别名
- Caspase-4
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背景
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Gene Symbols: CASP4
Protein Name (Synonyms): Caspase-4 (CASP-4) (EC 3.4.22.57) (ICE and Ced-3 homolog 2) (ICH-2) (ICE(rel)-II) (Mih1) (Protease TX) [Cleaved into: Caspase-4 subunit p10, Caspase-4 subunit p20
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基因ID
- 837
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UniProt
- P49662
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
抗原 See all Caspase 4 (CASP4) ELISA试剂盒
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