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Quick Overview for PLG ELISA 试剂盒 (ABIN8055941)
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See all PLG ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78.1 pg/mL
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原理
- Sandwich Human Plasminogen ELISA Kit to quantitate Human Plasminogen in cell culture supernatants, serum, plasma(heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Plasminogen (PLG) ELISA Kit
PLG 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: 小鼠 Colorimetric Competition ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: 大鼠 Colorimetric Competition ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: Cow Colorimetric Competition ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: 人 Colorimetric Competition ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: 兔 Colorimetric Competition ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: Cow Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen (PLG) ELISA KitPLG 适用: 犬 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5.8%, 5.4%, 7.2%
Inter-Assay Precision (CV%): 5.9%, 2.6%, 4.1% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- PLG (Plasminogen (PLG))
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别名
- PLG
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背景
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Background: PLAUR (PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. PLAUR is originally identified as a saturable binding site for urokinase on the cell surface. And the gene plays an important role in many normal as well as pathologic processes. The PLAUR gene is localized to 19q13.31. PLAUR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. PLAUR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus it seems to be an important player in the regulation of this process. In human coronary artery vascular smooth muscle cells, UPA stimulates cell migration via a UPAR signaling complex containing TYK2 and phosphatidylinositol 3-kinase.
Gene Full Name: plasminogen
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基因ID
- 5340
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UniProt
- P00747
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途径
- Complement System, Lipid Metabolism
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