Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit
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北京 101111
Quick Overview for Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit (ABIN7850029)
抗原
See all IVD 试剂盒检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 0.27 μmol/L - 5.00 μmol/L
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最低检测浓度
- 0.27 μmol/L
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原理
- Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit
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产品特性
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Acetyl-CoA is an essential cofactor and acyl carrier in the enzymatic acetyl transfer reaction. It can be formed by oxidative decarboxylation of pyruvate in mitochondria, oxidation of long-chain fatty acids, or oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound of the citric acid cycle.
The detection principle of this kit is that Acetyl-CoA is converted into other substances under the condition of enzyme catalysis, and the resulting fluorescence is detected by fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm.
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实验时间
- 0.5 h
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实验精密度
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Inter-assay precision: 2.7-3.6%
Intra-assay precision: 1.7-4.1%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- -20 °C
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储存方法
- This product can be stored at -20°C for 12 months with shading light.
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有效期
- 12 months
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- IVD (Isovaleryl-CoA Dehydrogenase (IVD))
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别名
- Coenzyme A
抗原
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