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- 抗原 See all IVD 试剂盒
- IVD (Isovaleryl-CoA Dehydrogenase (IVD))
- 应用范围
- Biochemical Assay (BCA)
- 特异性
- 3 μM fatty acid
- 产品特性
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Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 5 - 1000 µM, fluorimetric assay 3 - 100 µM CoA.
Convenient. Room temperature mix-and-read procedure can be readily automated for high-throughput assay of thousands of samples per day. - 组件
- Assay Buffer: 20 mL. Dye Reagent: 120 µL. Enzyme A: 120 µL. Enzyme B: 120 µL. Substrate: 600 µL. Standard: 50 µL ATP: 120 µL.
- 试剂未包括
- Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.
- Top Product
- Discover our top product IVD ELISA Kit
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Isovaleryl-CoA Dehydrogenase (IVD) ELISA Kit
IVD 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Assays: CoA in a variety of biological samples.
- 结果分析
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Subtract Blank value (Standard #4) from the standard values and plot the OD or F against standard concentrations. Note: if the calculated CoA concentration of a sample is higher than 1000 µM in the Colorimetric Assay or 100 µM in the Fluorimetric Assay, dilute sample in Assay Buffer and repeat the assay. Multiply result by the dilution factor, n.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
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- 抗原
- IVD (Isovaleryl-CoA Dehydrogenase (IVD))
- 别名
- Coenzyme A (IVD 产品)
- 别名
- 1300016K07Rik Kit, 6720455E18Rik Kit, AI463340 Kit, ACAD2 Kit, ATIVD Kit, ISOVALERYL-COA-DEHYDROGENASE Kit, ISOVALERYL-COA-DEHYDROGENASE PRECURSOR Kit, IVDH Kit, isovaleryl-CoA-dehydrogenase Kit, isovaleryl coenzyme A dehydrogenase Kit, isovaleryl-CoA dehydrogenase Kit, isovaleryl-CoA-dehydrogenase Kit, Ivd Kit, IVD Kit
- 背景
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Quantitative determination of coenzyme A (CoA) by colorimetric (570nm) or fluorimetric (530/590nm) method.
Procedure: 60 min.
Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of CoA's most crucial roles is the carrying and transferring of acyl groups. This method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.
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