Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
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北京 101111
Quick Overview for Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit (ABIN7849938)
抗原
See all ALT 试剂盒检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 0.01 U/L - 0.83 U/L
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最低检测浓度
- 0.01 U/L
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原理
- Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
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产品特性
- ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
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实验时间
- 1.5 h
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实验精密度
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Inter-assay precision: 9.8%
Intra-assay precision: 2.3%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- -20 °C
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储存方法
- This product can be stored at -20°C for 12 months with shading light.
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有效期
- 12 months
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- ALT (Alanine Aminotransferase (ALT))
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别名
- ALT
抗原
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