Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
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北京 101111
Quick Overview for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (ABIN5708826)
抗原
See all ALT 试剂盒检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 1.26 IU/L - 72.3 IU/L
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最低检测浓度
- 1.26 IU/L
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原理
- The Alanine Aminotransferase (ALT/GPT) Assay Kit (Reitman-Frankel method) is biochemical kit in Colorimetric method
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产品特性
- ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37 °C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
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试剂未包括
- Spectrophotometry (Visible range)
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实验时间
- 1.5 h
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实验精密度
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Inter-assay precision: 8.2%
Intra-assay precision: 4.3%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- 4 °C
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储存方法
- This product can be stored at 2~8°C for 12 months with shading light.
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有效期
- 12 months
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- ALT (Alanine Aminotransferase (ALT))
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别名
- ALT
抗原
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