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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

AcA Colorimetric Cell Samples, Plasma, Serum, Tissue Samples Quantitative
产品编号 ABIN5708826
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (ABIN5708826)

抗原

See all ALT 试剂盒
ALT (Alanine Aminotransferase (ALT))

检测方法

Colorimetric

应用范围

Activity Assay (AcA)

样品类型

Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method

Quantitative
  • 检测范围

    1.26 IU/L - 72.3 IU/L

    最低检测浓度

    1.26 IU/L

    原理

    The Alanine Aminotransferase (ALT/GPT) Assay Kit (Reitman-Frankel method) is biochemical kit in Colorimetric method

    产品特性

    ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37 °C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

    试剂未包括

    Spectrophotometry (Visible range)
  • 实验时间

    1.5 h

    实验精密度

    Inter-assay precision: 8.2%

    Intra-assay precision: 4.3%

    限制

    仅限研究用
  • 注意事项

    Protect from light.

    储存条件

    4 °C

    储存方法

    This product can be stored at 2~8°C for 12 months with shading light.

    有效期

    12 months
  • 抗原

    ALT (Alanine Aminotransferase (ALT))

    别名

    ALT
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