VEGFA ELISA 试剂盒
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Quick Overview for VEGFA ELISA 试剂盒 (ABIN7832885)
抗原
See all VEGFA ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
质量等级
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原理
- FastELISA VEGFA (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human VEGFA (Vascular Endothelial Growth Factor A) in Serum, plasma.
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Analytical Method
- Quantitative
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特异性
- This assay is highly specific for detection of Human VEGFA. No significant cross-reactivity or interference between Human VEGFA and analogues was observed.
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Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit
VEGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitHigh Sensitivity VEGFA 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Vascular Endothelial Growth Factor A (VEGFA) ELISA KitVEGFA 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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有效期
- 6 months
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGFA
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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