DDIT3 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for DDIT3 ELISA 试剂盒 (ABIN7831015)
抗原
See all DDIT3 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- DDIT3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of DDIT3 expression in cultured cells.
-
Analytical Method
- Qualitative
-
-
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA Kit
DDIT3 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 适用: 大鼠 Colorimetric Competition ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))
-
别名
- DDIT3
-
背景
-
Synonyms: CEBPZ|CHOP|CHOP10|GADD153|MGC4154
Gene Description: DNA-damage-inducible transcript 3
-
基因ID
- 1649
-
途径
- Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis
抗原 See all DDIT3 ELISA试剂盒
-
