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NEK9 ELISA 试剂盒

The 人 NEK9 ELISA Kit (ABIN7830984) is a Colorimetric ELISA Kit designed to quantify 人 NEK9.
产品编号 ABIN7830984
发货至: 中国
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Quick Overview for NEK9 ELISA 试剂盒 (ABIN7830984)

抗原

See all NEK9 ELISA试剂盒
NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

适用

  • 1
  • 1
  • 1

检测方法

Colorimetric

实验类型

Cell ELISA

应用范围

ELISA

样品类型

Cell Samples, Suspension Cell Culture
  • 原理

    NEK9 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NEK9 expression in cultured cells.

    Analytical Method

    Qualitative

    交叉反应

    小鼠, 大鼠
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Uncoated

    实验流程

    1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
    2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
    3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
    4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    Store at 4°C for six months.
  • 抗原 See all NEK9 ELISA试剂盒

    NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

    别名

    NEK9

    背景

    Synonyms: DKFZp434D0935|MGC138306|MGC16714|NERCC|NERCC1|Nek8

    Gene Description: NIMA (never in mitosis gene a)- related kinase 9

    基因ID

    91754

    UniProt

    Q8TD19

    途径

    SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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