RPS6KA1 ELISA 试剂盒
The 人 RPS6KA1 ELISA Kit (ABIN7830975) is a Colorimetric ELISA Kit designed to quantify 人 RPS6KA1.
产品编号 ABIN7830975
发货至:
中国
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Contact Info
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Quick Overview for RPS6KA1 ELISA 试剂盒 (ABIN7830975)
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RPS6KA1
(Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 expression in cultured cells.
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Analytical Method
- Qualitative
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Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) ELISA Kit
RPS6KA1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
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别名
- RPS6KA1
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背景
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Synonyms: HU-1|MAPKAPK1A|RSK|RSK1
Gene Description: ribosomal protein S6 kinase, 90 kDa, polypeptide 1
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基因ID
- 6195
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UniProt
- Q15418
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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