STAT5A ELISA 试剂盒
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Quick Overview for STAT5A ELISA 试剂盒 (ABIN7830904)
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See all STAT5A ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- STAT5A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of STAT5A expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA Kit
STAT5A 适用: 人, 大鼠, 小鼠 pTyr694 Colorimetric Cell ELISA Cell Culture Cells
Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA KitSTAT5A 适用: 人, 大鼠, 小鼠 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA KitSTAT5A 适用: 人 pTyr694 Colorimetric Sandwich ELISA Cell Culture Lysate
Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA KitSTAT5A 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA KitSTAT5A 适用: 人 pTyr694 Colorimetric Sandwich ELISA Cell Culture Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
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别名
- STAT5A
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背景
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Synonyms: MGF|STAT5
Gene Description: signal transducer and activator of transcription 5A
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基因ID
- 6776
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途径
- JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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