Src ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 Src.
产品编号 ABIN7830896
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Src ELISA 试剂盒 (ABIN7830896)
抗原
See all Src ELISA试剂盒
Src
(Proto-oncogene tyrosine-protein kinase Src (Src))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- SRC (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SRC expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Proto-oncogene tyrosine-protein kinase Src (Src) ELISA Kit
Src 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- Src (Proto-oncogene tyrosine-protein kinase Src (Src))
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别名
- SRC
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背景
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Synonyms: ASV|SRC1|c-SRC|p60-Src
Gene Description: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
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基因ID
- 6714
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途径
- JAK/STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
抗原 See all Src ELISA试剂盒
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