RPS6KB1 ELISA 试剂盒
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Quick Overview for RPS6KB1 ELISA 试剂盒 (ABIN7830883)
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检测方法
实验类型
应用范围
样品类型
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原理
- RPS6KB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RPS6KB1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
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别名
- RPS6KB1
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背景
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Synonyms: PS6K|S6K|S6K1|STK14A|p70(S6K)-alpha|p70-S6K|p70-alpha
Gene Description: ribosomal protein S6 kinase, 70 kDa, polypeptide 1
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基因ID
- 6198
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途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect
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