Patched 1 ELISA 试剂盒
The 人 Patched 1 ELISA Kit (ABIN7830864) is a Colorimetric ELISA Kit designed to quantify 人 Patched 1.
产品编号 ABIN7830864
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Patched 1 ELISA 试剂盒 (ABIN7830864)
抗原
See all Patched 1 (PTCH1) ELISA试剂盒
Patched 1 (PTCH1)
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- PTCH1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PTCH1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- Patched 1 (PTCH1)
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别名
- PTCH1
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背景
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Synonyms: BCNS|FLJ26746|FLJ42602|HPE7|NBCCS|PTC|PTC1|PTCH|PTCH11
Gene Description: patched homolog 1 (Drosophila)
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基因ID
- 5727
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途径
- Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
抗原 See all Patched 1 (PTCH1) ELISA试剂盒
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