PKC beta ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for PKC beta ELISA 试剂盒 (ABIN7830854)
抗原
See all PKC beta (PRKCB) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- PRKCB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKCB expression in cultured cells.
-
Analytical Method
- Qualitative
-
交叉反应
- 小鼠, 大鼠
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
-
别名
- PRKCB
-
背景
-
Synonyms: MGC41878|PKC-beta|PKCB|PRKCB1|PRKCB2
Gene Description: protein kinase C, beta
-
基因ID
- 5579
-
途径
- WNT signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling
抗原 See all PKC beta (PRKCB) ELISA试剂盒
-