SMAD3 ELISA 试剂盒
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Quick Overview for SMAD3 ELISA 试剂盒 (ABIN7830810)
抗原
See all SMAD3 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- SMAD3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SMAD3 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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SMAD, Mothers Against DPP Homolog 3 (SMAD3) ELISA Kit
SMAD3 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 3 (SMAD3) ELISA KitSMAD3 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 3 (SMAD3) ELISA KitSMAD3 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 3 (SMAD3) ELISA KitSMAD3 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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别名
- SMAD3
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背景
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Synonyms: DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396
Gene Description: SMAD family member 3
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基因ID
- 4088
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途径
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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