JAK3 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for JAK3 ELISA 试剂盒 (ABIN7830714)
抗原
See all JAK3 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- JAK3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JAK3 expression in cultured cells.
-
Analytical Method
- Qualitative
-
交叉反应
- 小鼠, 大鼠
-
-
Janus Kinase 3 (JAK3) ELISA Kit
JAK3 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Janus Kinase 3 (JAK3) ELISA KitJAK3 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Janus Kinase 3 (JAK3) ELISA KitJAK3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Janus Kinase 3 (JAK3) ELISA KitJAK3 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- JAK3 (Janus Kinase 3 (JAK3))
-
别名
- JAK3
-
背景
-
Synonyms: JAK-3|JAK3_HUMAN|JAKL|L-JAK|LJAK
Gene Description: Janus kinase 3 (a protein tyrosine kinase, leukocyte)
-
基因ID
- 3718
-
UniProt
- P52333
-
途径
- JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response
抗原 See all JAK3 ELISA试剂盒
-
