JAK2 ELISA 试剂盒
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Quick Overview for JAK2 ELISA 试剂盒 (ABIN7830713)
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检测方法
实验类型
应用范围
样品类型
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原理
- JAK2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JAK2 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Janus Kinase 2 (JAK2) ELISA Kit
JAK2 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Janus Kinase 2 (JAK2) ELISA KitJAK2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Janus Kinase 2 (JAK2) ELISA KitJAK2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- JAK2 (Janus Kinase 2 (JAK2))
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别名
- JAK2
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背景
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Synonyms: JTK10
Gene Description: Janus kinase 2 (a protein tyrosine kinase)
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基因ID
- 3717
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UniProt
- O60674
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途径
- JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response
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