Integrin beta 3 ELISA 试剂盒
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Quick Overview for Integrin beta 3 ELISA 试剂盒 (ABIN7830702)
抗原
See all Integrin beta 3 (ITGB3) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- ITGB3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ITGB3 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠
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Integrin beta 3 (ITGB3) ELISA Kit
ITGB3 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Integrin beta 3 (ITGB3) ELISA KitITGB3 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Integrin beta 3 (ITGB3) ELISA KitITGB3 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Integrin beta 3 (ITGB3) ELISA KitITGB3 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- Integrin beta 3 (ITGB3)
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别名
- ITGB3
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背景
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Synonyms: CD61|GP3A|GPIIIa
Gene Description: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
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基因ID
- 3690
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UniProt
- P05106
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途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex
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