Integrin beta 3 ELISA 试剂盒
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- 抗原 See all Integrin beta 3 (ITGB3) ELISA试剂盒
- Integrin beta 3 (ITGB3)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Integrin beta 3/CD61 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Integrin beta 3.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ITGB3 ELISA Kit
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Integrin beta 3 (ITGB3) ELISA Kit
ITGB3 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Integrin beta 3 (ITGB3) ELISA KitITGB3 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Integrin beta 3 (ITGB3) ELISA KitITGB3 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Integrin beta 3 (ITGB3) ELISA试剂盒
- Integrin beta 3 (ITGB3)
- 别名
- Integrin beta 3 (CD61 (ITGB3 产品)
- 别名
- BDPLT16 ELISA Kit, BDPLT2 ELISA Kit, CD61 ELISA Kit, GP3A ELISA Kit, GPIIIa ELISA Kit, GT ELISA Kit, INGRB3 ELISA Kit, integrin ELISA Kit, INTB3 ELISA Kit, GPIIIA ELISA Kit, cd61 ELISA Kit, gp3a ELISA Kit, itgb3 ELISA Kit, itgb3.2 ELISA Kit, itgb3.1 ELISA Kit, integrin subunit beta 3 ELISA Kit, integrin beta 3 ELISA Kit, integrin subunit beta 3 L homeolog ELISA Kit, integrin beta 3a ELISA Kit, integrin beta 3b ELISA Kit, ITGB3 ELISA Kit, Itgb3 ELISA Kit, itgb3.L ELISA Kit, itgb3a ELISA Kit, itgb3b ELISA Kit
- UniProt
- Q9Y6R9
- 途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex
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