GSK3 alpha ELISA 试剂盒
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Quick Overview for GSK3 alpha ELISA 试剂盒 (ABIN7830690)
抗原
See all GSK3 alpha (GSK3a) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- GSK3A/GSK3B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GSK3A/GSK3B expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA Kit
GSK3a 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA KitGSK3a 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA KitGSK3a 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA KitGSK3a 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA KitGSK3a 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
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别名
- GSK3A
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背景
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Synonyms: DKFZp686D0638
Gene Description: glycogen synthase kinase 3 alpha
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基因ID
- 2931
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UniProt
- P49840
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途径
- PI3K-Akt Signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect
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