FANCD2 ELISA 试剂盒
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Quick Overview for FANCD2 ELISA 试剂盒 (ABIN7830642)
抗原
See all FANCD2 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- FANCD2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FANCD2 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- FANCD2 (Fanconi Anemia, Complementation Group D2 (FANCD2))
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别名
- FANCD2
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背景
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Synonyms: DKFZp762A223|FA-D2|FA4|FACD|FAD|FAD2|FANCD|FLJ23826
Gene Description: Fanconi anemia, complementation group D2
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基因ID
- 2177
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UniProt
- Q9BXW9
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途径
- DNA Damage Repair
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