H2AFX ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for H2AFX ELISA 试剂盒 (ABIN7830577)
抗原
See all H2AFX ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- H2AFX (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of H2AFX expression in cultured cells.
-
Analytical Method
- Qualitative
-
交叉反应
- 小鼠, 大鼠
-
-
H2A Histone Family, Member X (H2AFX) ELISA Kit
H2AFX 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
H2A Histone Family, Member X (H2AFX) ELISA KitH2AFX 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- H2AFX (H2A Histone Family, Member X (H2AFX))
-
别名
- H2AFX
-
背景
-
Synonyms: H2A.X|H2A/X|H2AX
Gene Description: H2A histone family, member X
-
基因ID
- 3014
-
UniProt
- P16104
-
途径
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原 See all H2AFX ELISA试剂盒
-
